Introduction to One Drop of Body Fluid - PCR (1DF-PCR)

mRNA Profiling: Body Fluid Identification Using Multiplex Real-Time PCR

An Introduction to Real-Time PCR

The development of instruments that allowed real-time monitoring of fl uorescence within PCR reaction vessels was a very signifi cant advance. The technology is very flexible and many alternative instruments and fl uorescent probe systems have been developed and are currently available. Real-time PCR assays can be completed very rapidly since no manipulations are required post-amplifi cation. I...

Aspergillus PCR: one step closer to standardization.

PCR has been used as an aid in the diagnosis of invasive aspergillosis for almost 2 decades. A lack of standardization has limited both its acceptance as a diagnostic tool and multicenter clinical evaluations, preventing its inclusion in disease-defining criteria. In 2006, the European Aspergillus PCR Initiative was formed. The aim of the initiative was to provide optimal standardized protocols...

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*

Real-time PCR in Food Science: Introduction 25 Real-time PCR in Food Science: Introduction

Food safety and quality control programmes are increasingly applied throughout the production food chain in order to guarantee added value products as well as to minimize the risk of infection for the consumer. The development of real-time PCR has represented one of the most significant advances in food diagnostics as it provides rapid, reliable and quantitative results. These aspects become in...